Salesforce also backed the round, which brought the company’s fundraising total to $66.5m.
Skyhigh said it will use the funds invest in further product innovation and support its international growth.
The round follows Skyhigh’s $20m Series B raised in May 2013 and backed by Greylock and Sequoia.
Greylock partner Asheem Chandna said, “As employees across the globe embrace the cloud, organizations have become increasingly hungry for a service that provides comprehensive visibility and control.
“Skyhigh Networks is the clear market leader in cloud security due to its strong customer momentum across global 2,000 companies, its leading technology platform, and its world-class team.”
Greylock closed its latest fund on $1bn last September, four years after raising a similar amount for its thirteenth vehicle.
The firm’s previous investment include LinkedIn, Facebook and Workday.
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