The company said the capital will be used towards setting up 25 new centres over the next two years.
Rajasthan-based ASG added it plans to build its footprint in Jharkhand, Madhya Pradesh, Uttar Pradesh, Chhattisgarh and Bihar.
Sequoia Capital India Advisors managing director Abhay Pandey said, “ASG Eye Hospitals brings the best technology in healthcare to Tier II and Tier III markets and offers advanced and competent eye care services in the region.”
Sequoia Capital manages about $1.4bn focused on India.
To date it has invested in more than 50 companies in the region including Café Coffee Day, Idea Cellular, Just Dial, Manappuram Finance, Paras, Quick Heal, Micromax, Mu Sigma and Vasan Health Care. The firm has offices in Bangalore, Mumbai and New Delhi.
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